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KMID : 0370219850290010050
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Yakhak Hoeji
1985 Volume.29 No. 1 p.50 ~ p.54
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Determination of Chloramphenicol in Milk by High Performance Liquid Chromatography
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김경례/Kim KR
김정한/최경숙/Kim JH/Choi KS
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Abstract
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Seven different sorbents were evaluated for their adsorptivity and desorptivity of antibiotic, chloramphenicol. Among the sorbents studied, Carbopak B was found to be the most efficient in enriching the chloramphenicol from dilute aqueous solution. Interfering components in the milk matrix could be washed off by water and petroleum ether from Carbopak B column, while the chloramphenicol was retained on the surface of Carbopak B. The method of simple and efficient purification and enrichment of chloramphenicol using Carbopak B, followed by quantitative analysis employing C18 reversed phase high performance liquid chromatography has been applied to the determination of chloramphenicol in milk.
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